Plugins / FLIMKit-Anisotropy / Inputs · edit on GitHub
Inputs
- A parallel and a perpendicular PTU acquisition, selected explicitly rather than inferred.
- A separate measured IRF for each polarisation channel, as a LAS X export.
- A fluorescence lifetime from an independent analysis, which the fit holds fixed.
- G and relative exposure factors, which the fit also holds fixed.
Optional: subpixel registration between the two acquisitions, spatial pooling, photon thresholds and validity masks.